EXPLORATION OF BEAUVERIA BASSIANA ENTOMOPATHOGEN ON LEPTOCORISA ACUTA IN RICEFIELD
Article History
Submited : October 26, 2022
Published : December 9, 2022
Keywords:
Beauveria bassiana (Bals.) Vuill is a fungus that infecting the insect Leptocorisa oratorius F. naturally in ricefields. This study aimed to obtain isolates of the entomopathogenic fungus B. bassiana from the insects body of infected L. oratorius, which would later be used as biological agents in controlling rice pests. The exploratory research was carried out at rice production centers in Sidera Village, Sigi Regency and in Dolago Village, Parigi-Moutong Regency, Central Sulawesi. The laboratory tests were carried out at the Plant Disease Laboratory, Faculty of Agriculture, Tadulako University. Research methods include exploring L. oratorius insects infected by B. bassiana in the field, and then the specimens were brought to the laboratory for isolation and identification, calculation of conidia density, colony relative growth rate, and determination of viability. The results showed that L. oratorius was infected with the fungus B. bassiana in Sidera and in Dolago. Isolates from both regions had the same macroscopic and microscopic morphological characteristics, but had different characteristics of conidia density, colony relative growth rate, and viability.
Aregger, E., 1992. Conidia Production of The Fungus Beauveria brongniartii On Barley and Quality Evaluation During Storage At 2°C. Journal of Invertebrate Pathology. 59(1):2-10. J.I.P. 59, 2–10.
Arsi, A., Pujiastuti, Y., Kusuma, S.S.H., Gunawan, B., 2020. Eksplorasi, isolasi dan identifikasi Jamur entomopatogen yang menginfeksi serangga hama. J. Prot. Trop. Crop 1, 70.
Barnett, H.L., Hunter, B.B., 1972. Illustrated Genera of Imperfect Fungi, Third Edition. ed. Burgess Publishing, Minnesota.
Boucias, D.G., Pendland, J.C., 1998. Principles of Insect Pathology. Kluwer Academic Publishers, Massachusetts (US).
DPPKP, 2014. Pedoman Uji Mutu dan Uji Efikasi Lapangan Agens Pengendali Hayati (APH). Direktorat Perlindungan Perkebunan Kementerian Pertanian, Jakarta.
Dwiastuti, M.E., Kurniawati, M.Y., 2007. Keefektifan Entomopatogen Hirsutella citriformis (Deuteromycetes: Moniliales) pada Kutu Psyllid Diaphorina citri Kuw. J.Hort 17, 244–252.
Effendy, T.A., Septiadi, R., Salim, A., Mazid, A., 2010. Entomopathogenic Fungi from The Lowland Soil of South Sumatera and Their Potential As Biocontrol Agents of Stink Bug (Leptocorisa oratorius (F.)). J. HPT Tropika 10, 154–160.
Fuxa, J.R., Richter, A.R., 2004. Effects of Soil Moisture and Composition and Fungal Isolate on Prevalence of Beauveria bassiana in Laboratory Colonies of the Red Imported Fire Ant (Hymenoptera: Formicidae). Environ Entomol 33, 975–981.
Gabriel, B.P., Riyanto, 1989. Metarhizium anisopliae (Metsch.) Sor. Taksonomi, Patologi, Produksi, dan Aplikasinya. Proyek pengembangan perlindungan tanaman perkebunan Departemen Pertanian.
Gandjar, I., Samson, R.A., Vermeulen, K.V.D.T., Oetari, A., Santoso, I., 1999. Pengenalan Kapang Tropik Umum. Yayasan Obor, Jakarta.
Goettel, M.S., Inglis, D.G., 1997. Fungi: Hyphomycetes., in: Manual of Techniques in Insect Pathology (L. Lacey, Ed.). Academic Press, London, pp. 213–249.
Hasyim, A., Azwana, 2003. Patogenisitas Isolat Beauveria bassiana (Balsamo) Vuillemin dalam Mengendalikan Hama Penggerek Bonggol Pisang, Cosmopolites sordidus Germar. J.Hort. 13, 120–130.
Herdatiarni, F., Himawan, T., Rachmawati, R., 2014. Eksplorasi Cendawan Entomopatogen Beauveria sp. menggunakan Serangga Umpan Pada Komoditas Jagung, Tomat dan Wortel Organik Di Batu, Malang. J. HPT 1, 1–11.
Herlinda, S., Mulyati, S.I., Suwandi, 2008. Selection of Isolates of Entomopathogenic Fungi and the Bioefficacy of Their Liquid Production against Leptocorisa oratorius Nymphs. MIcrobiol Indones 2, 141–146.
Herlinda, S., Sari, E.M., Pudjiastuti, Y., Suwandi, Nurnawati, E., Riyanta, A., 2005. Variation in Virulence of Beauveria bassiana (Bals.) Vuill. Strains on Larvae of Plutella xylostella (L.) (Lepidoptera: Plutellidae). Agritop. 24, 52-57.
Herlinda, S., Utama, M.D., Pujiastuti, Y., Suwandi, S., 2006. Density and Viability of Spores of Beauveria bassiana (Bals.) Vuill. Due To Sub-Cultures and Media Enriched, and Its Virulence Against Larvae of Plutella xylostella (Linn.). J. HPT Tropika 6, 70–78.
Jauharlina, Hendrival, 2003. Toksisitas (LC50 dan LT50) Jamur Entomopatogen Beauveria bassiana (Bals.) Vuill. Terhadap Hama Ulat Grayak (Spodoptera litura F.). Agrista 7, 295–302.
Jeniesthiana, J., 2011. Effect of Growth Media and Long Storage to The Viability of Beauveria bassiana (Bals.) Vuill. Universitas Jember.
McCoy, C.W., Samson, R.A., Boucias, D.G., 1988. Entomogenous Fungi. In: Ignoffo, C.M. and Mandava, N.B., Eds., Handbook of Natural Pesticides, Vol. V, Microbial Insecticides, Part A,. Boca Raton, Florida.
Minarni, E., Soesanto, L., Suyanto, A., Rostaman, R., 2020. Exploration and pathogenicity test of Entomopathogenic fungus from brown plant hopper (Nilaparvata lugens Stal) Pest. Eco. Env. & Cons. 26, 24–33.
Minarti, M., 2018. Culture of Beauveria bassiana and Its Efficacy Againts Nymph of Nilaparvata lugens (Stal.) (Homoptera: Delphacidae). Universitas Sriwijaya, Palembang.
Nuraida, Hasyim, A., 2009. Isolation, Identification, and Characterization of Entomopathogenic Fungi from Rhizosphere of Cabbage Plant. J. Hort. 19, 419–432.
Permadi, M., Lubis, R., Sari, D., 2018. Eksplorasi Cendawan Entomopatogen Dari Berbagai Rizosfer Tanaman Hortikultura Di Beberapa Wilayah Kabupaten Mandailing Natal Provinsi Sumatera Utara. Agritech 20, 23–32.
Prayogo, Y.W., Tengkano, Marwoto, 2005. Prospect of Entomophatogenic Fungus Metarhizium Anisopliae To Control Spodoptera Litura On Soybean. J. Litbangtan 24, 19–26.
Ristiari, N., Julyasih, K., Suryanti, I., 2018. Isolasi dan Identifikasi Jamur Mikroskopis pada Rizosfer Tanaman Jeruk Siam (Citrus nobilis Lour.) Di Kecamatan Kintamani, Bali. JPBU 6, 10–19.
Rohman, F.L., Saputro, T.B., Prayogo, Y., 2017. Pengaruh Penambahan Senyawa Berbasis Kitin Terhadap Pertumbuhan Cendawan Entomopatogen Beauveria bassiana. JSSITS 6, 2337–3520.
Samson, R.A., Evans, H.C., Latge, J.P., 1988. Atlas of entomopathogenic fungi. . Prinejer verlag Berlin Heodelberg, New York. London. Tokyo.
Sari, W., Rosmeita, C., 2020. Identifikasi Morfologi Cendawan Entomopatogen Beauveria bassiana dan Metarhizium anisopliae Asal Tanaman Padi Cianjur. Jurnal Pro-Stek 2, 1–9.
Soetopo, D., Indrayani, I., 2007. Status Teknologi dan Prospek Beauveria bassiana untuk Pengendalian Serangga Hama Tanaman Perkebunan yang Ramah Lingkungan. Perspektif 6, 29–46.
Suharto, Trisusilowati, E.B., Purnomo, H., 1998. Study on Physiological Aspects of Beauveria bassiana and Their Virulence to Helicoverpa armigera. JPTI 4, 112–119.
Tanada, Y., Kaya, H.K., 1993. Insect Pathology. Academic Press Inc., San Diego, New York, Boston, London, Sydney, Tokyo, Toronto.
Trizelia, 2005. The Entomopathogenic Fungus Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hypomycetes): Genetic Variability, Physiological Chracterization, and Its Virulence To Crocidolomia Pavonana (F.) (Lepidoptera Paralidae). IPB, Bogor.
Utami, R.S., Isnawati, Ambarwati, R., 2014. Eksplorasi dan Karakterisasi Cendawan Entomopatogen Beauveria bassiana dari Kabupaten Malang dan Magetan. LenteraBio 3, 59–66.
Arsi, A., Pujiastuti, Y., Kusuma, S.S.H., Gunawan, B., 2020. Eksplorasi, isolasi dan identifikasi Jamur entomopatogen yang menginfeksi serangga hama. J. Prot. Trop. Crop 1, 70.
Barnett, H.L., Hunter, B.B., 1972. Illustrated Genera of Imperfect Fungi, Third Edition. ed. Burgess Publishing, Minnesota.
Boucias, D.G., Pendland, J.C., 1998. Principles of Insect Pathology. Kluwer Academic Publishers, Massachusetts (US).
DPPKP, 2014. Pedoman Uji Mutu dan Uji Efikasi Lapangan Agens Pengendali Hayati (APH). Direktorat Perlindungan Perkebunan Kementerian Pertanian, Jakarta.
Dwiastuti, M.E., Kurniawati, M.Y., 2007. Keefektifan Entomopatogen Hirsutella citriformis (Deuteromycetes: Moniliales) pada Kutu Psyllid Diaphorina citri Kuw. J.Hort 17, 244–252.
Effendy, T.A., Septiadi, R., Salim, A., Mazid, A., 2010. Entomopathogenic Fungi from The Lowland Soil of South Sumatera and Their Potential As Biocontrol Agents of Stink Bug (Leptocorisa oratorius (F.)). J. HPT Tropika 10, 154–160.
Fuxa, J.R., Richter, A.R., 2004. Effects of Soil Moisture and Composition and Fungal Isolate on Prevalence of Beauveria bassiana in Laboratory Colonies of the Red Imported Fire Ant (Hymenoptera: Formicidae). Environ Entomol 33, 975–981.
Gabriel, B.P., Riyanto, 1989. Metarhizium anisopliae (Metsch.) Sor. Taksonomi, Patologi, Produksi, dan Aplikasinya. Proyek pengembangan perlindungan tanaman perkebunan Departemen Pertanian.
Gandjar, I., Samson, R.A., Vermeulen, K.V.D.T., Oetari, A., Santoso, I., 1999. Pengenalan Kapang Tropik Umum. Yayasan Obor, Jakarta.
Goettel, M.S., Inglis, D.G., 1997. Fungi: Hyphomycetes., in: Manual of Techniques in Insect Pathology (L. Lacey, Ed.). Academic Press, London, pp. 213–249.
Hasyim, A., Azwana, 2003. Patogenisitas Isolat Beauveria bassiana (Balsamo) Vuillemin dalam Mengendalikan Hama Penggerek Bonggol Pisang, Cosmopolites sordidus Germar. J.Hort. 13, 120–130.
Herdatiarni, F., Himawan, T., Rachmawati, R., 2014. Eksplorasi Cendawan Entomopatogen Beauveria sp. menggunakan Serangga Umpan Pada Komoditas Jagung, Tomat dan Wortel Organik Di Batu, Malang. J. HPT 1, 1–11.
Herlinda, S., Mulyati, S.I., Suwandi, 2008. Selection of Isolates of Entomopathogenic Fungi and the Bioefficacy of Their Liquid Production against Leptocorisa oratorius Nymphs. MIcrobiol Indones 2, 141–146.
Herlinda, S., Sari, E.M., Pudjiastuti, Y., Suwandi, Nurnawati, E., Riyanta, A., 2005. Variation in Virulence of Beauveria bassiana (Bals.) Vuill. Strains on Larvae of Plutella xylostella (L.) (Lepidoptera: Plutellidae). Agritop. 24, 52-57.
Herlinda, S., Utama, M.D., Pujiastuti, Y., Suwandi, S., 2006. Density and Viability of Spores of Beauveria bassiana (Bals.) Vuill. Due To Sub-Cultures and Media Enriched, and Its Virulence Against Larvae of Plutella xylostella (Linn.). J. HPT Tropika 6, 70–78.
Jauharlina, Hendrival, 2003. Toksisitas (LC50 dan LT50) Jamur Entomopatogen Beauveria bassiana (Bals.) Vuill. Terhadap Hama Ulat Grayak (Spodoptera litura F.). Agrista 7, 295–302.
Jeniesthiana, J., 2011. Effect of Growth Media and Long Storage to The Viability of Beauveria bassiana (Bals.) Vuill. Universitas Jember.
McCoy, C.W., Samson, R.A., Boucias, D.G., 1988. Entomogenous Fungi. In: Ignoffo, C.M. and Mandava, N.B., Eds., Handbook of Natural Pesticides, Vol. V, Microbial Insecticides, Part A,. Boca Raton, Florida.
Minarni, E., Soesanto, L., Suyanto, A., Rostaman, R., 2020. Exploration and pathogenicity test of Entomopathogenic fungus from brown plant hopper (Nilaparvata lugens Stal) Pest. Eco. Env. & Cons. 26, 24–33.
Minarti, M., 2018. Culture of Beauveria bassiana and Its Efficacy Againts Nymph of Nilaparvata lugens (Stal.) (Homoptera: Delphacidae). Universitas Sriwijaya, Palembang.
Nuraida, Hasyim, A., 2009. Isolation, Identification, and Characterization of Entomopathogenic Fungi from Rhizosphere of Cabbage Plant. J. Hort. 19, 419–432.
Permadi, M., Lubis, R., Sari, D., 2018. Eksplorasi Cendawan Entomopatogen Dari Berbagai Rizosfer Tanaman Hortikultura Di Beberapa Wilayah Kabupaten Mandailing Natal Provinsi Sumatera Utara. Agritech 20, 23–32.
Prayogo, Y.W., Tengkano, Marwoto, 2005. Prospect of Entomophatogenic Fungus Metarhizium Anisopliae To Control Spodoptera Litura On Soybean. J. Litbangtan 24, 19–26.
Ristiari, N., Julyasih, K., Suryanti, I., 2018. Isolasi dan Identifikasi Jamur Mikroskopis pada Rizosfer Tanaman Jeruk Siam (Citrus nobilis Lour.) Di Kecamatan Kintamani, Bali. JPBU 6, 10–19.
Rohman, F.L., Saputro, T.B., Prayogo, Y., 2017. Pengaruh Penambahan Senyawa Berbasis Kitin Terhadap Pertumbuhan Cendawan Entomopatogen Beauveria bassiana. JSSITS 6, 2337–3520.
Samson, R.A., Evans, H.C., Latge, J.P., 1988. Atlas of entomopathogenic fungi. . Prinejer verlag Berlin Heodelberg, New York. London. Tokyo.
Sari, W., Rosmeita, C., 2020. Identifikasi Morfologi Cendawan Entomopatogen Beauveria bassiana dan Metarhizium anisopliae Asal Tanaman Padi Cianjur. Jurnal Pro-Stek 2, 1–9.
Soetopo, D., Indrayani, I., 2007. Status Teknologi dan Prospek Beauveria bassiana untuk Pengendalian Serangga Hama Tanaman Perkebunan yang Ramah Lingkungan. Perspektif 6, 29–46.
Suharto, Trisusilowati, E.B., Purnomo, H., 1998. Study on Physiological Aspects of Beauveria bassiana and Their Virulence to Helicoverpa armigera. JPTI 4, 112–119.
Tanada, Y., Kaya, H.K., 1993. Insect Pathology. Academic Press Inc., San Diego, New York, Boston, London, Sydney, Tokyo, Toronto.
Trizelia, 2005. The Entomopathogenic Fungus Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hypomycetes): Genetic Variability, Physiological Chracterization, and Its Virulence To Crocidolomia Pavonana (F.) (Lepidoptera Paralidae). IPB, Bogor.
Utami, R.S., Isnawati, Ambarwati, R., 2014. Eksplorasi dan Karakterisasi Cendawan Entomopatogen Beauveria bassiana dari Kabupaten Malang dan Magetan. LenteraBio 3, 59–66.
Yunus, M., Salmirna, S., & Edy, N. (2022). EXPLORATION OF BEAUVERIA BASSIANA ENTOMOPATHOGEN ON LEPTOCORISA ACUTA IN RICEFIELD. AGROLAND The Agricultural Sciences Journal (e-Journal), 9(2), 107 - 116. https://doi.org/10.22487/agroland.v9i2.1475
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