Identifikasi Molekuler Bakteri Resisten Logam Berat dari Ekosistem Limbah Batubara untuk Aplikasi Bioremediasi

Abstrak

The existence of heavy metals in the environment is a very important pollution problem that can give rise to problems and instability in ecology. Bioremediation is a technique that can be used to degrade waste metal. The mechanisms of biosorption and bioaccumulation will not influence bacterial viability. The study aims to acquire and identify, in a way, molecular indigenous bacteria from waste that can potentially reduce heavy metal or bioaccumulation. The method covers isolation and screening of bacteria-resistant heavy metals, identification of morphology, and analysis of DNA sequences, as well as reduction tests for heavy metals. The results of the study show 10 isolates of bacteria originating from waste coal were gram-positive, and two isolates of bacteria selected that were IF6 and IF9, were capable of reducing the metal weight of Zn, Pb, and Cd at a concentration of 25 ppm. Conclusion based on identification using 16S rRNA gene sequence shows that isolate IF6 m has an index similarity of 100% with Kocuria flava, and IF9 has similarity with species Fictibacillus nanhensis. This hope can be used to know the ability of indigenous microbes originating from waste coal to degrade heavy metals.